Scientist/Sr. Scientist Biochemist (Blue Sky Initiative)

at St. Jude Children's Research Hospital in Memphis, Tennessee, United States

Job Description

We are looking for multiple experienced biochemists to aid in structural studies of protein kinases. The biochemists will join an inter-disciplinary Blue Sky Initiative on “Seeing the Invisible in Protein Kinases”, which is supported by $35M in institutional funding by St. Jude Children’s Research Hospital. The project will extend the ultra-high field NMR spectroscopy and biochemistry approaches developed recently by the Kalodimos lab (Science 2020, 370, eabc2754) to study the human kinome. This is a large-scale project that aims to detect and structurally characterize the distinct conformational states populated by kinases, and to dissect the mechanisms of allosteric regulation and those underpinning oncogenic and drug-resistance mutations. We expect this initiative to have a profound impact on the field, transforming our understanding of protein kinases.

Preferred skills and experiences

+ Experience with molecular biology and molecular cloning techniques including subcloning, site-directed mutagenesis, PCR and DNA manipulation is desired.

+ Experience with a variety of protein expression platforms, including E. coli, insect cell culture and mammalian expression systems.

+ Expert in protein purification by various chromatographic techniques including but not limited to IMAC, IEC, HIC, SEC etc.

+ Experience with protein engineering or rational design of recombinant proteins along with characterization techniques (for example MS, SEC-MALs, DSF, DLS, SDS-Page etc.).

+ Strong track record of scientific accomplishments demonstrated through publications in highly regarded journals.

+ Outstanding organizational and multi-tasking skills; able to manage several projects going simultaneously.

+ Maintain familiarity with current scientific literature relevant to the research experiments or project.

+ An excellent team-player with strong communication skills and a willingness to mentor others.

St. Jude Children’s Research Hospital offers a remarkable opportunity to perform cutting edge research with outstanding institutional support and exceptional shared core facilities in an environment of open collaboration, educational advancement, and training opportunities. St. Jude is consistently ranked on Fortune Magazine’s and Glassdoor’s “100 Best Places to Work” for companies with 1,000 or more employees. We are located in the heart of Memphis, Tennessee, a vibrant and friendly city at the historic American crossroads of music, trade, food, and culture. Mild winters and beautiful green outdoor spaces are part of the reasons why Forbes recently named Memphis the 4th happiest city in which to work. Cost of living and high quality of life are other attractions; Money magazine listed Memphis as one of the least expensive cities in which to raise a child. St. Jude facilities and salaries are highly competitive.

+ Performs tasks related to the research project independently, but within specific guidelines and subject to regular review by supervisor.

+ Independently develop and implement construct design, protein purification and biophysical characterization of proteins.

+ Leverage deep knowledge and hands-on experience with versatile protein purification technologies using established chromatography, FPLC, and HPLC platforms and other analytical methods to ensure the highest quality of lead proteins.

+ Manages and trains staff as appropriate in laboratory activities.

+ Present problems and propose solutions in discussions with group members. Offer insights based on experience to group members to help solve existing problems.

+ Work effectively in a collaborative team environment where results are achieved through influence, cooperation, and the incorporation of multiple points of view.

+ Bachelor’s degree in relevant scientific area is required.


+ Seven (7) years of relevant post-degree work experience is required

+ Five (5) years of relevant post-degree work experience is required with a Master’s degree

+ Three (3) years of relevant post-degree work experience is required with a PhD

Senior Scientist

+ Ten (10) years relevant post-degree work experience is required

+ Eight (8) years of relevant post-degree work experience is required with a Master’s degree

+ Six (6) years of relevant post-degree work experience is required with a PhD

COVID-19 vaccine:

As of September 10, 2021, St. Jude Children’s Research Hospital has mandated the COVID-19 vaccine for all employees, excluding those with an approved medical or religious accommodation, as a condition of employment.

EEO Statement: St. Jude is an Equal Opportunity Employer

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Posted Job Title: Scientist/Sr. Scientist Biochemist (Blue Sky Initiative)

WITS Req ID: 44582

Posted Department: Structural Biology

Street: 262 Danny Thomas Place

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Job Posting: JC185232606

Posted On: Jun 07, 2021

Updated On: Dec 05, 2021